GLI1 and glioma: Therefore from our analysis it can be easily assumed that perturbation (i.e. mutation, malfunction, high or low expression etc.)of this protein or node in Hedgehog signaling network will affect the normal network function and may cause several types of cancers, which is also found in experimental observation, as in Glioma, Colon and Pancreatic cancer cell lines GLI1 shows over expression [52], [72], [85].