Perturbing at a time the combinations of the cytoplasmic activated proteins GLI1, GLI2 and membrane protein SMO in Glioma scenario; SMO, HFU, ULK3 and RAS in Colon cancer scenario; SMO, HFU, ULK3, RAS and ERK12 in Pancreatic cancer scenario; we observed the under expressions of various oncoproteins in Hedgehog pathway. The gene discussed is SMO; the disease is central nervous system cancer.