We found that, if we perturbed the logical states of SMO, GLI1 and GLI2 proteins from 1 to 0 in Glioma model, it will be possible to suppress the expression of various output proteins (e.g. JAGGED2, WNT, SFRP, CYCLIN_B, CYCLIN_D, CYCLIN_D2, CYCLIN_E, OPN, SNAI1, CMYC, BMI, BCL2, FOXM1, PDGFRΑ etc.)as well as the phenotypic expressions of the Glioma affected cell (Figure 4). The gene discussed is SPP1; the disease is glioma.