Also, in Glioma scenario, we identified that the activation pathways (solid green arrows in Figure 8D) from RAS, ERK12, ULK3 and TWIST to GLI1 could up regulate this protein, whereas the activation pathways (solid green arrows in Figure 8E) from RAS, ULK3 to GLI1 in Colon cancer scenario or the activation pathways (solid green arrows in Figure 8F) from RAS, ULK3 and ERK12 to GLI1 in pancreatic cancer scenario could also be the alternative pathways to up regulate the GLI1 protein. Here, TWIST1 is linked to central nervous system cancer.