As several experimental studies demonstrate that over activation of GLI proteins in glial stem cell lines is the main cause of Glioma formation [52], [57], we propose that the over activation of GLI protein in Glioma happens due to the effect of the higher concentration of SHH as well as the intracellular activation or deregulation by HFU, ULK3, RAS, TWIST, ERK12 proteins in cytoplasm. This evidence concerns the gene SHH and central nervous system cancer.