In order to prevent such collateral damage, we propose that, in Glioma cell line, selectively targeting SMO in cell membrane, and the nuclear translocation of activated GLI1 and GLI2 within cytoplasm would be more effective to completely shut down the Hedgehog pathway by suppressing the activity of different proteins responsible for uncontrolled cellular proliferation (Figure 4) and this was found to be the minimal combinations of proteins required. This evidence concerns the gene GLI1 and glioma.