Twenty-four hours following ablation, pmel T cells were infused i.v. We then performed an ELISpot assay to measure the frequency of pmel T cells within spleens (Figure 6A) and tumor-draining lymph nodes (TDLN) (Figure 6B) 9 days after adoptive transfer (day 20) by measuring IFN-γ release in response to hgp100. This evidence concerns the gene IFNG and neoplasm.