LOX IMVI human melanoma cells were transfected with either pGL3b-hPTCH1-prom-wt (Patched promoter containing 2 wild type GLI1 binding sites) or pGL3b-hPTCH1-prom-mut (Patched promoter containing 2 mutated GLI1 binding sites) and both containing the luciferase reporter sequence. The gene discussed is GLI1; the disease is melanoma.