To investigate whether beclin-1 and LC3 could be predictive for the overall survival of hypopharyngeal squamous cell carcinoma patients, the Kaplan-Meier analysis was performed, which showed that the overall survival rates of patients with beclin-1 negative tumors was significantly lower than of patients with beclin-1 positive tumors (P<0.0001) (Figure 3A). This evidence concerns the gene BECN1 and hypopharynx squamous cell carcinoma.