The presence of Single Nucleotide Polymorphisms in the p53 gene, used as a marker for aflatoxin exposure [36] were also investigated in the GLCS subjects using Restriction Fragment Length Polymorphism Polymerase Chain Reaction and this showed the presence of at least one mutation in 4.4% of controls, 12.4% of LC’s and 36.2% of subjects diagnosed with HCC. This evidence concerns the gene TP53 and laryngotracheoesophageal cleft.