The present study confirmed that LPS stimulates iNOS and COX-2, which was associated with overexpression of NF-κB, whereas orally administrated DZO greatly reduces the expression of iNOS and COX-2 and hence the expression of NF-κB. It is likely that the anti-inflammatory activity of DZO contributes to the reduced expression of iNOS and COX-2 in LPS-induced liver injury. Here, NOS2 is linked to injury.