To further consolidate our hypothesis that KME inhibits obesity, we evaluated the effects of KME treatment in vitro using an adipocyte differentiation development system, which is regulated by transcriptional activators such as PPAR-γ, C/EBP-α, and some transcriptional regulators at the molecular level [39, 40]. This evidence concerns the gene PPARG and obesity due to melanocortin 4 receptor deficiency.