Thus, at least for mating pairs involving the Fn/Sat parental line for which the kinetics of hybrid formation in P. duboscqi was investigated, there was a significant correlation between the probability of hybrid recovery and the number of days of co-infection (Fn/Sat×Lv39/Hyg cross, p = 0.024; Fn/Sat×Sd/Hyg cross, p = 0.034; Fig S2). This evidence concerns the gene FN1 and coinfection.