APOE and Familial prostate cancer: The intensities of the overlapping 35/38-bp bands were lower for all the Apoε2/ε4 restriction fragments because of the absence of the 35 bp fragment in all Apoε2 genotypes.Fig. 1shows the polyacrylamide gel separation of ApoE isoforms from genomic DNA of prostate cancer cells, after the DNA amplification by PCR, and digestion with the restriction enzymeHhaI. The digested fragments revealed the presence of homozygous and heterozygous combinations of Apoε alleles.