For example, deletions in EGFR exon 19 and SNVs in EGFR exon 18 aa719, exon 20 aa768 and exon 21 aa858 in lung adenocarcinoma [8]; KRAS SNVs in exon 2 aa12 in 50% of wildtype EGFR lung adenocarcinoma [14] and BRAF mutations at exon 15 aa600 in DNA from malignant melanoma [15] were all accurately identified by NGS, concordant with conventional mutation detection methods. This evidence concerns the gene KRAS and melanoma.