To confirm protein levels in the mammary tumors, western blotting was performed for IGF-IR, Akt1, Akt2, phosphorylated Akt (pAkt), Erk1/2, phosphorylated Erk1/2 (pErk1/2), Stat3 and phosphorylated Stat3 (pStat3). Here, AKT1 is linked to breast cancer.