Due to the complex composition and high cell heterogeneity of bone marrow, researchers analyzed serum samples of different hematologic malignancies in recent years using 2-DE combined with MALDI-TOF-MS or Protein chip-SELDI-TOF-MS, for instance, Albitar [6], Zou [7] and Mohamedali [8] had established the diagnostic model of leukemia with high sensitivity and specificity, and further identified the leukemia-related markers, such as Rho-GDP dissociation inhibitor autoantibodies, alpha enolase, aldolase enzyme A and so on [9]. This evidence concerns the gene ENO1 and leukemia.