Moreover, the apoptotic effect of N-AChE-S was abolished by small interfering RNA (siRNA) against AChE, cholinesterase inhibitors, apoptosis inhibitors, and by transfection of the anti-apoptotic Bcl proteins.17,18 A key player in apoptosis and Alzheimer’s disease is glycogen synthase kinase 3 (GSK3).19,20 We therefore investigated whether N-AChE-S affects GSK3 activation. Here, ACHE is linked to early-onset autosomal dominant Alzheimer disease.