A previousin vivostudy of the effects of NM 0.5% on xenograft tumor growth of osteosarcoma MNNG-HOS cells in nude mice support these results in that it demonstrated significant inhibition of xenograft tumor growth (53%, p=0.0001), tumor vascularity and VEGF and MMP-9 tumor tissue staining compared to tumors of mice in the control diet group(35). This evidence concerns the gene MMP9 and osteosarcoma.