A significant negative correlation was found between NM modulation of Matrigel invasion inhibition and MMP-2 secretion with osteosarcoma MNNG-HOS (r=−0.6531), osteosarcoma U-2OS (r=−0.835) and rhabdomyosarcoma RD (r=−0.675). The gene discussed is MMP2; the disease is rhabdomyosarcoma.