Upon treatment of MDA-MB-468 breast cancer cells with epidermal growth factor (EGF), confocal immunofluorescence revealed that EGFR underwent retrograde movement to the Golgi and the ER (endoplasmic reticulum) where the N-terminus was within the lumen of the Golgi/ER and the C-terminus was exposed to the cytoplasm (66). This evidence concerns the gene EGF and breast cancer.