To help fill this research gap, this study investigated the relationship and nature of interaction among ENO1, plasminogen, uPA, and uPAR; the effect of blocking ENO1 with Ab or silencing the expression of ENO1 with a specific shRNA plasmid in lung cancer cells; and the impact of adoptive transfer of Ab against ENO1 on the establishment of lung and bone metastasis by lung cancer cells in animal models. This evidence concerns the gene PLG and lung cancer.