Within three days following transient expression of a silencing vector targeting LB1 (shLB1) in the human osteosarcoma cell line U-2 OS, LB1 protein expression decreased by ∼75–80% as determined by immunoblotting; and its mRNA level was reduced by ∼65% as shown by qRT-PCR analyses (Fig. 1A, B). Here, CKAP2 is linked to osteosarcoma.