To determine the incidence and clinical spectrum of germline TP53 mutations in adult-onset sarcoma populations, a systematic screen using multiplexed ligation-dependent probe amplification and Sanger sequencing was undertaken in 559 probands consecutively recruited from adult sarcoma clinics–agnostic to family history–on the Australian arm of the International Sarcoma Kindred Study (ISKS; http://www.anzctr.org.au; http://www.australiansarcomagroup.org/sarcomakindredstudy/index.html). This evidence concerns the gene TP53 and sarcoma.