In the present study, firstly, we demonstrated the inhibitory effect of IPA-3 on FBS- and TNF-α-induced phosphorylation of PAK1 in HCC cell lines, which was consistent with the earlier findings that IPA-3 impeded both basal and PDGF-stimulated PAK1 activation in fibroblasts [8]. The gene discussed is PAK1; the disease is hepatocellular carcinoma.