In conclusion, the present study demonstrated that CD-3 could inhibit the proliferation of HCC cell line in-vitro and suppresses HCC tumor growth in-vivo and consequently induced caspase-dependent apoptosis via up-regulation of p53, bax and down-regulation of bcl-2, AP-1 and NF-κB. This evidence concerns the gene NFKB1 and hepatocellular carcinoma.