To determine whether the CDF mediated inhibition of growth of CR colon cancer could partly be attributed to restoration of miR-21-PTEN-Akt axis, we have examined (a) the relationship between miR-21 and PTEN and (b) the effect of CDF on the expression of miR-21, PTEN and activation of Akt axis in different colon cancer cells. This evidence concerns the gene AKT1 and colonic neoplasm.