PLAGL1 and cancer: Based on the magnitude and consistency of hypermethylation across multiple CpG sites, the strongest evidence for disrupted methylation patterns at imprinted genes was demonstrated at five tumor suppressor genes: DLK1, PLAGL1, SLC22A18, TP73, and WT1. Of the five genes, LOI has been reported for WT1 in Wilms’ tumor development, [23] and has not been reported for the other four genes in the context of cancer development.