ACSS1 and Hypoglycemia: In this regard, studies of AceCS2-deficient mice revealed that AceCS2 is specifically required for metabolic homeostasis when the mice are fed a low carbohydrate/high fat diet (LC/HFD); AceCS2-deficient animals are essentially normal on a chow diet but show poor weight gain, hypothermia, hypoglycemia, and impaired survival on an LC/HFD (Sakakibara et al., 2009).