VEGF and MMP-9 production that are associated with angiogenesis and metastasis was significantly decreased in a dose dependent manner in HCT 116 colon cancer cells by STB-HO as shown in Figure 4A and Figure 4B. Also, though further changing medium one day later, the production of VEGF and MMP-9 was still suppressed in HCT 116 cancer cells, implying that STB-HO may exert anti-angiogenic activity in cancer cells. This evidence concerns the gene MMP9 and malignant colon neoplasm.