IFNB1 and infection: To determine if IFN gene expression differed between influenza-infected NHBE or NSBE cells, the two cell types were infected at a low multiplicity of infection (MOI) of 0.01 with human (A/NY/55/2004, H3N2), swine (A/swine/MN/02749/2009, H1N1), or avian influenza viruses (A/chicken/TX/167280-04/2002, H5N3; A/chicken/PA/13609/1993, H5N2; A/mute swan/MI/451072-2/2006, H5N1) and IFNα, IFNβ, and IFNλ gene expression determined at 8 and 24 hours post-infection (HPI) (Figure 1).