We observed that 48 hours of pretreatment with EPA resulted in a statistically significant increase of CK20 expression at the mRNA and protein levels (p<0.05 and p<0.01, respectively) and in a decrease in CD133 mRNA expression (p<0.05) that was consistently and significantly associated with a lowered IC25 and IC50 of the bulk of tumor COLO 320 DM cells to either oxaliplatin or 5-Fluorouracil monotherapy. This evidence concerns the gene PROM1 and neoplasm.