Cytoplasmic FOXO1 expression in high-grade glioma was significantly higher than that of low-grade glioma (median H-score: 80 versus 10, P = 0.005) while nuclear FOXO1 expression was comparable in both (median H-score: 40 versus 40, P = 0.471) (Fig. 1A, B). This evidence concerns the gene FOXO1 and central nervous system cancer.