To determine if GATA1 is overexpressed in AMKL compared to other subtypes of AML in non-DS children, real-time RT-PCR was performed to quantify transcript levels of GATA1 in a cohort of diagnostic AML blast samples (12 AMKL cases and 31 non-AMKL AML cases). Here, GATA1 is linked to acute megakaryoblastic leukemia.