POU5F1 and neoplasm: According to the results of the IHC assay of 64 tumor specimens, CD44 and CD133 were located in the cytomembrane with a positive rate of 72% (46/64) and 70% (45/64),respectively; Oct4 was expressed in the nucleus and the positive rate was 79.7% (51/64); Sox2 was distributed in the nucleus with a positive rate of 81.3% (52/64); the major part of p-AKT was located in cytoplasm with a minor distribution in the nucleus, and the positive rate was 93.8% (60/64); p-ERK was majorly distributed in the nucleus with a minor part in cytoplasm, and the positive rate was 64% (41/64).