The present study was undertaken to investigate whether Snail expression in areca quid chewing-associated OSCCs, and further to test the effects of ROS generation and snail expression by arecoline, the major areca nut alkaloid, in human oral keratinocytes (HOKs) and oral cancer cell line OECM-1 by using DCFH-DA assay and Western blot. Here, SNAI1 is linked to lip and oral cavity carcinoma.