To determine if spectra percentage differences observed for Plin 2 on LF- and HF-CLD reflected actual abundance differences, we investigated the effects of LF and HF-refeeding on hepatic Plin2 by confocal immunofluorescence (IF) microscopy, and by quantitative immunoblot analysis of isolated CLD (Figure 5). Here, SLC26A3 is linked to congenital secretory chloride diarrhea 1.