To determine if spectra percentage differences observed for Plin 2 on LF- and HF-CLD reflected actual abundance differences, we investigated the effects of LF and HF-refeeding on hepatic Plin2 by confocal immunofluorescence (IF) microscopy, and by quantitative immunoblot analysis of isolated CLD (Figure 5). This evidence concerns the gene PLIN2 and congenital secretory chloride diarrhea 1.