After observing methylated NTRK3 in the colorectal cancers run on the HumanMethylation450 arrays, we assessed the methylation status of NTRK3 in a second independent set of normal colon mucosa, colon adenomas, and CRCs using a quantitative methylation-specific PCR assay (qMSP; MethyLight) designed to assess the promoter region of NTRK3. We first established that a Percent of Methylated Reference (PMR) threshold of 13.7% had a specificity of ∼90% for cancer vs. normal tissue. Here, NTRK3 is linked to cancer.