NTF3 and malignant colon neoplasm: Therefore, we assessed the expression levels of NT-3 in the panel of colon cancer cell lines previously assessed for methylated NTRK3. No NT-3 expression was detected in RKO, HCT116, FET, Vaco400 and HT-29, whereas NT-3 was expressed at a low (although relatively high level in relation to the other CRC cell lines) in SW480.