E2F1 and central nervous system cancer: The consistent and dose-dependent reduction of luciferase activity was observed following miR-329 transfection in both glioma cells, the reporter assay revealed that the repressive effect of miR-329 on the luciferase activity of E2F1 3′UTR was abolished by miR-329 inhibitor but did not have the effect in the miR-329 mut group (Figure 4D).