The result of colony formation assay showed overexpressing E2F1 significantly increased the proliferation rate of LN18 and T98G glioma cells compared with that cells expressing E2F1-3′UTR (Figure 5A), the rescuing experiment further confirmed that the inhibitory role of miR-329 in glioma cells may be mediated by E2F1. This evidence concerns the gene E2F1 and central nervous system cancer.