CD86 and neoplasm: In order to determine if pretreatment with sTGF-βR affects anti-tumor CTLs indirectly through interruption of these 4 steps, we used flow cytometry to study the effect of pretreatment with sTGF-βR on both the number of DCs and the expression of DC-activation markers (CD86, MHC class I and II) in the tumor and TDLNs.