ACKR3 and central nervous system cancer: LN229 and LN308 glioma cells infected with shRNA vector directed against CXCR7 or LacZ were exposed to SDF-1α for 15 min in the presence or absence of 100 nM of AMD3100 and analyzed for total and phosphorylated ERK1/2 and Akt by Western blot analysis (Figure 5).