In vivo colocalization of Iba1 with EGFP-(viral antigen) positive cells demonstrated that RSA59 can directly infect microglia but that does not confirm that infected microglia were resident microglia because in intracranial (IC) inoculation blood brain barrier can be disrupted and blood monocytes/macrophages can migrate and acquire infection. The gene discussed is AIF1; the disease is infection.