GHRL and obesity due to melanocortin 4 receptor deficiency: Male adult C57BL/6 mice, with a normal weight and with diet-induced obesity (DIO), were randomized into six weight matched groups (n=6/group) and each group of mice received three intra-peritoneal injections with two weeks intervals, containing either 75 μg of ghrelin-NS1 immunoconjugate, 75 μg of NS1 or PBS.