Furthermore, antibodies titres attained after the immunization protocol were not very high, when compared to antibodies titres after common infectious diseases, which is also reassuring in safety concerns, since complete neutralization of ghrelin was not the purpose of an anti-ghrelin vaccination strategy for obesity treatment as ghrelin also intervenes in several key biological processes besides appetite regulation, such as growth hormone secretion, gastro-intestinal and cardiovascular functions [29]. This evidence concerns the gene GH1 and obesity due to melanocortin 4 receptor deficiency.