The decision to use diet induced obesity mice (DIO) that develop obesity in result of the sedentary lifestyle and increased caloric intake, resided in the fact that this obesity animal model is the one that more accurately reflects the most common cause of obesity in order to allow the evaluation of the interaction between ghrelin and anorexigenic pathways, which compared to the ob/ob or the db/db mouse, which present monogenetic causes of obesity that are rare in the human setting. Here, GHRL is linked to obesity due to melanocortin 4 receptor deficiency.