Present results on bio-imaging of EGFR with EGF-NIR in COLO205 further extend the applicability of this probe for estimation of the level of EGFR in CRC derived cell lines and are in accordance with a previous study in which cytotoxicity of EGFR-targeted immunoliposomes mediated specific and efficient drug delivery according to EGFR expression levels in COLO205 and HT-25 CRC and other cell lines [42]. This evidence concerns the gene EGF and colorectal carcinoma.