In the frozen sections, the BRCA1 antibody staining showed punctate, intra-nuclear granules in varying numbers of tumor, lactating, and normal cells In the FFPE tissue, we found reduced BRCA1 nuclear staining in breast tumor tissue compared to normal tissue, and less BRCA1 staining with higher histological grade in the tumors. This evidence concerns the gene BRCA1 and breast neoplasm.