To confirm the interaction of CD44 with moesin on HA-treatment, we performed co-IP assays with antibodies specific for CD44 / moesin using whole cell lysates obtained from glioma cells (U87 / U373) treated with either tumor necrosis factor-α (TNF-α, 10 nM for 24 h) or HA (100 μg/mL, 48 h), and no treatment control (NTC). Here, TNF is linked to central nervous system cancer.