Multiple regression analysis identified three independent variables presenting a significant association with definite PBC: high avidity anti-PDC-E2 antibodies (OR 4.12; 95 % CI 2.12–8.02), high titer IIF-AMA antibodies (OR 4.89; 95 % CI 2.32–10.31), and three or more cell domains recognized by autoantibodies (OR 9.41; 95 % CI 1.92–46.06). This evidence concerns the gene DLAT and primary biliary cholangitis.