This allowed us to conclude that in silico estimates like these can reach an acceptable accuracy level for their practical consideration by cancer and neurodegeneration researches once the preference of these miRNAs for the proteins in the Argonaute family has become known, and so have yet unknown values of the affinity of any miRNA for two of the four proteins (50%), Ago1 and Ago4, which is absolutely required for a more accurate approximation. This evidence concerns the gene AGO1 and cancer.