As presented in Figure 6, Treg cell population from the DLN, spleen, and tumor was significantly decreased in mice treated with RdB/IL23/p35 as compared with mice treated with either RdB/IL12 or RdB/IL23 (P<0.01), suggesting that increased number of IFN-γ- and TNF-α-co-secreting T cells in RdB/IL23/p35-administraed mice may contribute to the reduction of Treg cell population. This evidence concerns the gene TNF and neoplasm.