To further confirm whether RdB/IL23/p35 mediates up-regulation of antitumor cytokines (IFN-γ and TNF-α) secreted by tumor-specific immune cells, we co-cultured splenocytes with irradiated B16-F10 or NIH3T3 for 3 days in the presence of recombinant human IL-2. Here, TNF is linked to neoplasm.