In addition, we reported that several estrogen-stimulated genes, including serpin peptidase inhibitor, clade B, member 11 (SERPINB11) [15], SERPINB3[16], cathepsin B (CTSB) [17], S-adenosylhomocysteine hydrolase-like protein 1 (AHCYL1)[18], alpha 2 macroglobulin (A2M) [19], secreted phosphoprotein 1 (SPP1)[20], pleiotrophin (PTN)[21], several cell cycle genes [22] and beta-defensin 11 (AvBD-11)[23] in the chicken oviduct are detected predominantly in glandular epithelial cells of ovaries from laying hens with ovarian adenocarcinoma. The gene discussed is SPP1; the disease is ovarian adenocarcinoma.