ANXA5 and ovarian carcinoma: Phosphatidylserine translocation was assessed to determine apoptosis in EA-treated ovarian carcinoma cells by staining with FITC-conjugated annexin V. Annexin V-positive ES-2 cells increased under treatment with more than 25 μM EA (Figure 3(a); P < 0.05), whereas PA-1 cells increased under treatment with 10 μM EA (Figure 3(b)), compared with untreated cells.