Firstly, protein levels for MMP2, MMP14, TIMP1, and TIMP2 in lung tissue samples homogenated with EDTA-free buffer were evaluated by Western blot under reducing and denaturing conditions to determine whether differences in gene expression translated into differences in protein expression in the fetal rabbit model for CDH undergoing TO (Figure 7). This evidence concerns the gene TIMP2 and congenital diaphragmatic hernia.