Fig 6A showed that knockdown of PTEN gene with siRNA prevented the insulin resistance upon prolonged exposure to GH (p<0.05). Similarly, over-expressing C124S disrupted insulin resistance upon prolonged GH exposure (p<0.05) (Fig. 6B). To the contrary, cells overexpressing wtPTEN had similar even slightly greater insulin resistance upon prolonged GH exposure (p<0.05) (Fig. 6C). This evidence concerns the gene PTEN and Insulin resistance.