The possibility that 5-FU is inducing an immunogenic tumor cell death/apoptosis is not likely given that it has been previously shown that treatment of EL4 cells (a non-OVA expressing predecessor to E.G7) with 5-FU did not increase surface expression of calreticulin, nor were the anti-tumor effects of 5-FU found to be TLR4-dependent [23]. Here, TLR4 is linked to neoplasm.