At 3 h incubation time, the expressional levels of RIP-1 in C6 glioma cells induced by 3.0 μmol/L and 6.0 μmol/L shikonin were 3.12±0.39 times and 5.49±1.25 times as high as that in the control group, respectively. The gene discussed is RIPK1; the disease is glioma.