As figure 3A showed, after 3 hours incubation with 3.0 μmol/L and 6.0 μmol/L shikonin, the necrosis (Annexin V−/Pl+) rates of C6 glioma cells were 25.0% and 35.89%, and late apoptosis (Annexin V+/Pl+) rates were 26.02% and 37.32%, respectively. Here, ANXA5 is linked to central nervous system cancer.