Additionally, pretreatment with Nec-1 made the C6 glioma cells at the stage of late apoptosis reduce from 26.02% to 13.60% at 3 μmol/L shikonin and from 37.32% to 24.17% at 6 μmol/L shikonin; made U87 cells at the stage of late apoptosis decrease from 33.37% to 7.96% at 5 μmol/L shikonin and from 42.90% to 27.14% at 10 μmol/L shikonin. This evidence concerns the gene PCSK1 and glioma.