EGF and breast adenocarcinoma: After 30 min of incubation, the compound significantly decreased phospho form of EGFR which further decreased with time upto 3 h in both MDA-MB 231 cells(p<0.05 to p<0.001) and primary breast adenocarcinoma cells (p<0.01 to p<0.001) while 100 ng/ml of EGF induced tyrosine phosphorylation of EGFR (p<0.01).